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The crystal structures of the N-domain from P12-1, a serine proteinase from Pseudomonas aeruginosa, reveal structural changes during activation and inactivation.
The crystal structures of the N-domain from a Ser proteinase of Pseudomonas aeruginosa have been solved at 2.2 A resolution. The crystal structure of the N-domain has two alpha-helices (residues 46-70 and 71-94), a beta-sheet (residues 35-44, 53-56 and 63-67), and three short turns (residues 45-49, 48-50 and 49-52). The protein is activated by the removal of a 9-residue peptide, Gln47-Lys53. The crystal structure of the inactive form reveals that the N-domain is locked in the active conformation. Furthermore, an ATP molecule is bound to the active site of the protein. The crystal structure of the N-domain of the inactive form of the protein reveals structural changes that must occur to expose the substrate binding cleft and to cause the molecule to be locked in the active conformation.A year ago, the old wooden fence at the corner of 9th and Lawrence was covered in bright, rainbow-colored tributes.

The rainbow flag had flown at the corner as a symbol of the gay community for decades, but on May 26, 2016, it was ripped down by the owner of the building, who had received a complaint that it was an eyesore.

Now, as the southern sector of Portland’s LGBTQ community gears up to mark its annual pride festival, the rainbow flag is back in its place, flying over what is now a thriving restaurant and cafe.

On Monday, Portland police officer Tyler Collins, also known as the Drag King Patrolman, and his partner, Jake Gustin, walked down the stairs of one of the restaurant’s two storefronts, where they had called in a report of a person being assaulted.

The team found the beaten-up flag and escorted it back up the stairs to the sidewalk, where the owner came outside and was visibly emotional.

“I’m so happy,” owner Mai Huynh said of her flag’s return. “It’s so important.”

Huynh said that when she bought the corner storefront a few months ago, she had to clear the fence that went around the corner. She started a petition to have the flag

 

2020.12.17 09:57
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2020.10.22 11:41 .
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Citrix Systems, has launched Remote Works, a new virtual series designed to share tips and best practices for staying engaged and productive.
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2020.10.22 11:41 .
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